To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Attenuated glial reaction… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Attenuated glial reactions and photoreceptor degeneration after retinal detachment in mice deficient in glial fibrillary acidic protein and vimentin.

Journal article
Authors Toru Nakazawa
Masumi Takeda
Geoffrey P Lewis
Kin-Sang Cho
Jianwei Jiao
Ulrika Wilhelmsson
Steven K Fisher
Milos Pekny
Dong F Chen
Joan W Miller
Published in Investigative ophthalmology & visual science
Volume 48
Issue 6
Pages 2760-8
ISSN 0146-0404
Publication year 2007
Published at Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
Pages 2760-8
Language en
Keywords Animals, Blotting, Western, Chemokine CCL2, metabolism, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Extracellular Signal-Regulated MAP Kinases, metabolism, Fluorescent Antibody Technique, Indirect, Glial Fibrillary Acidic Protein, deficiency, physiology, Gliosis, etiology, metabolism, prevention & control, In Situ Nick-End Labeling, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Microglia, physiology, Monocytes, physiology, Neuroglia, physiology, Photoreceptors, Vertebrate, pathology, Proto-Oncogene Proteins c-fos, metabolism, Retina, pathology, Retinal Degeneration, etiology, metabolism, prevention & control, Retinal Detachment, complications, Reverse Transcriptase Polymerase Chain Reaction, Vimentin, deficiency, physiology
Subject categories Neurobiology


PURPOSE: To characterize the reactions of retinal glial cells (astrocytes and Müller cells) to retinal injury in mice that lack glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) and to determine the role of glial cells in retinal detachment (RD)-induced photoreceptor degeneration. METHODS: RD was induced by subretinal injection of sodium hyaluronate in adult wild-type (WT) and GFAP-/-Vim-/- mice. Astroglial reaction and subsequent monocyte recruitment were quantified by measuring extracellular signal-regulated kinase (Erk) and c-fos activation and the level of expression of chemokine monocyte chemoattractant protein (MCP)-1 and by counting monocytes/microglia in the detached retinas. Immunohistochemistry, immunoblotting, real-time quantitative polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA) were used. RD-induced photoreceptor degeneration was assessed by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) and measurement of outer nuclear layer (ONL) thickness. RESULTS: RD-induced reactive gliosis, characterized by GFAP and vimentin upregulation, Erk and c-fos activation, MCP-1 induction, and increased monocyte recruitment in WT mice. Absence of GFAP and vimentin effectively attenuated reactive responses of retinal glial cells and monocyte infiltration. As a result, detached retinas of GFAP-/-Vim-/- mice exhibited significantly reduced numbers of TUNEL-positive photoreceptor cells and increased ONL thickness compared with those of WT mice. CONCLUSIONS: The absence of GFAP and vimentin attenuates RD-induced reactive gliosis and, subsequently, limits photoreceptor degeneration. Results of this study indicate that reactive retinal glial cells contribute critically to retinal damage induced by RD and provide a new avenue for limiting photoreceptor degeneration associated with RD and other retinal diseases or damage.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?