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Inhibition of NF-kappaB activation and chemokine expression by the leukocyte glycoprotein, CD43, in colon cancer cells.

Journal article
Authors Sirle Laos
Dan Baeckström
Gunnar C. Hansson
Published in International journal of oncology
Volume 28
Issue 3
Pages 695-704
ISSN 1019-6439
Publication year 2006
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 695-704
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Active Transport, Cell Nucleus, drug effects, Antigens, CD43, genetics, metabolism, Blotting, Western, Cell Line, Tumor, Cell Nucleus, drug effects, metabolism, Chemokine CCL2, genetics, metabolism, Chemokines, genetics, metabolism, Chemokines, CXC, genetics, metabolism, Colonic Neoplasms, genetics, metabolism, pathology, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Gene Expression Regulation, Neoplastic, genetics, Humans, I-kappa B Proteins, metabolism, Intercellular Signaling Peptides and Proteins, genetics, metabolism, Interleukin-8, genetics, metabolism, NF-kappa B, antagonists & inhibitors, genetics, metabolism, PPAR gamma, metabolism, RNA, Messenger, genetics, metabolism, Time Factors, Transcription Factor RelA, genetics, metabolism, Tumor Necrosis Factor-alpha, pharmacology
Subject categories Medical and Health Sciences

Abstract

CD43 is a heavily O-glycosylated type I trans-membrane protein, expressed at high levels on the surface of leukocytes. It is frequently overexpressed in early colon adenomas, but not in normal colon epithelial cells. To identify CD43 target genes, gene array analysis was performed using a tetracycline-inducible CD43 expression system in human colon adenocarcinoma SW480 cells. CD43 was demonstrated to down-regulate a variety of chemokine genes. Overexpression of CD43 suppressed constitutive as well as PMA-induced NF-kappaB activation and reduced the DNA binding of transcription factor p65 but not p50. Furthermore, a reduced NF-kappaB responsive promoter activity was observed and a decreased expression of proinflammatory chemokines MCP-1, IL-8 and GRO-alpha. These results suggest that overexpression of CD43 suppresses a subset of NF-kappaB target genes, partly via the inhibition of p65 transcriptional activity.

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