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Sulfatide with different … - University of Gothenburg, Sweden Till startsida
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Sulfatide with different fatty acids has unique distributions in cerebellum as imaged by time-of-flight secondary ion mass spectrometry (TOF-SIMS).

Journal article
Authors Zarah Pernber
Katrin Richter
Jan-Eric Månsson
Håkan Nygren
Published in Biochimica et biophysica acta
Volume 1771
Issue 2
Pages 202-9
ISSN 0006-3002
Publication year 2007
Published at Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 202-9
Language en
Keywords Animals, Cerebellum, metabolism, ultrastructure, Fatty Acids, analysis, Immunohistochemistry, Purkinje Cells, metabolism, Rats, Rats, Sprague-Dawley, Spectrometry, Mass, Secondary Ion, methods, Sulfoglycosphingolipids, chemistry, metabolism
Subject categories Medical and Health Sciences


In order to elucidate the biological role of sulfatide it is important to define the cellular and subcellular distribution of its various molecular species (e.g. fatty acid chain length and hydroxylation). We determined sulfatide species distribution in the rat cerebellum using time-of-flight secondary ion mass spectrometry (TOF-SIMS). TOF-SIMS detects ions up to m/z 10000 and enables simultaneous imaging of the lateral distribution of substances on an exposed surface, in this case a section through cerebellum. In addition to TOF-SIMS we analyzed sulfatide distribution in rat cerebellum using a sulfatide monoclonal antibody and confocal laser scanning microscopy. In the white matter, TOF-SIMS showed a uniform distribution of sulfatide with short chain fatty acids and a patchy distribution of sulfatide with C24 fatty acids. These patches had a low cholesterol signal. The granular layer showed a more uniform distribution of the sulfatide species, with the highest signal of C24. The molecular layer and Purkinje cells were devoid of sulfatide signals. Immunofluorescence showed the highest intensity in the white matter, lower intensity in the granular layer and absence of fluorescence in the molecular layer and Purkinje cells. The results are discussed in relation to previously published data and possible functional roles.

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