To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Coupling of antigen to ch… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Coupling of antigen to cholera toxin for dendritic cell vaccination promotes the induction of MHC class I-restricted cytotoxic T cells and the rejection of a cognate antigen-expressing model tumor.

Journal article
Authors Kristina Eriksson
Jia-Bin Sun
Inger Nordström
Margareta Fredriksson
Marianne Lindblad
Bin-Ling Li
Jan Holmgren
Published in European journal of immunology
Volume 34
Issue 5
Pages 1272-81
ISSN 0014-2980
Publication year 2004
Published at Institute of Internal Medicine, Dept of Rheumatology and Inflammation Research
Institute of Medical Microbiology/Immunology
Pages 1272-81
Language en
Links dx.doi.org/10.1002/eji.200324368
Keywords Animals, Antigens, immunology, pharmacology, Cholera Toxin, immunology, pharmacology, Dendritic Cells, immunology, Histocompatibility Antigens Class I, immunology, Immunotoxins, immunology, pharmacology, Mice, Neoplasms, drug therapy, immunology, T-Lymphocytes, Cytotoxic, drug effects, immunology, Vaccines, immunology, pharmacology
Subject categories Microbiology in the medical area

Abstract

We previously demonstrated that cholera toxin (CT) is highly efficient as a combined carrier and adjuvant for dendritic cell (DC) vaccination, inducing strong Th1-dominated B cell and CD4(+) T cell responses. In this study we show that vaccination with DC pre-pulsed ex vivo with CT-conjugated OVA (OVA-CT) gives rise to OVA-specific CD8(+) T cells that produce IFN-gamma and are cytotoxic for OVA-expressing E.G7 tumor cells both in vitro and in vivo. The induction of specific CD8(+) CTL by OVA-CT-treated DC was associated with enhanced presentation of OVA peptide (SIINFEKL) on MHC class I in combination with an overall activation of the pulsed DC. Vaccination of mice with OVA-CT-pulsed DC resulted in rejection of already established MHC class I-positive, MHC class II-negative, OVA-expressing E.G7 tumors in an antigen-specific, CD8(+) T cell-dependent fashion and was associated with high numbers of tumor-infiltrating CD8(+) T cells. Conjugation of antigen to CT facilitated DC uptake of the linked antigen through the GM1 receptor-binding B subunit and induced strong activation-maturation signals through the biologically active A subunit. These results have interesting implications for DC vaccination aimed at inducing CTL immune responses.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?