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Characterization of T-cell reactive epitopes in glycoprotein G of herpes simplex virus type 2 using synthetic peptides.

Journal article
Authors Lars Bellner
Gun-Britt Löwhagen
Petra Tunbäck
Inger Nordström
Jan-Åke Liljeqvist
Kristina Eriksson
Published in Archives of virology
Volume 150
Issue 7
Pages 1393-406
ISSN 0304-8608
Publication year 2005
Published at Institute of Selected Clinical Sciences, Department of Dermatology and Venereology
Institute of Internal Medicine, Dept of Rheumatology and Inflammation Research
Institute of Laboratory Medicine, Dept of Clinical Virology
Pages 1393-406
Language en
Links dx.doi.org/10.1007/s00705-005-0497-...
Keywords Adult, Antibodies, Viral, chemistry, CD4-Positive T-Lymphocytes, immunology, Epitopes, immunology, Female, Herpesvirus 2, Human, immunology, Humans, Male, Viral Envelope Proteins, immunology
Subject categories Microbiology in the medical area

Abstract

We have previously shown that the CD4+ T-cell response to herpes simplex virus type 2 glycoprotein G-2 is type-specific and can thus be used to evaluate herpes simplex virus type 2-specific T-cell responses in individuals with a concomitant herpes simplex virus type 1 infection. In this study we have followed the glycoprotein G-2-specific T-cell responses over time, and also tried to identify T-cell epitopes in the membrane bound portion and the secreted portion of glycoprotein G-2 using synthetic peptides spanning the whole amino acid sequence of glycoprotein G-2. We found that the magnitude of the glycoprotein G-2-specific response varied considerably in infected individuals over time, even though all patients responded to at least one of the two glycoproteins at all time-points examined. We could also document strong T-cell responses to synthetic peptides from the secreted glycoprotein G-2 but only low responses to synthetic peptides corresponding to sequences from the heavily glycosylated membrane-bound glycoprotein G-2. We were able to map an immunogenic region (amino acid 31-125) within the secreted glycoprotein G-2. This region of the glycoprotein induced proliferative responses in 47% of the herpes simplex virus type 2-infected individuals. However, we were not able to identify any universal T-cell epitope.

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