To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Glycoprotein I of herpes … - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Glycoprotein I of herpes simplex virus type 1 contains a unique polymorphic tandem-repeated mucin region.

Review article
Authors Peter Norberg
Sigvard Olofsson
Mads Tarp Agervig
Henrik Clausen
Tomas Bergström
Jan-Åke Liljeqvist
Published in The Journal of general virology
Volume 88
Issue Pt 6
Pages 1683-8
ISSN 0022-1317
Publication year 2007
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 1683-8
Language en
Links dx.doi.org/10.1099/vir.0.82500-0
Keywords Glycosylation, Herpesvirus 1, Human, chemistry, genetics, metabolism, Immunoblotting, N-Acetylgalactosaminyltransferases, metabolism, Polymorphism, Genetic, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Repeat Sequences, Viral Envelope Proteins, chemistry, genetics, metabolism
Subject categories Medical and Health Sciences

Abstract

Glycoprotein I (gI) of herpes simplex virus type 1 (HSV-1) contains a tandem repeat (TR) region including the amino acids serine and threonine, residues that can be utilized for O-glycosylation. The length of this TR region was determined for 82 clinical HSV-1 isolates and the results revealed a polymorphic distribution of two to six or eight repeated blocks with a majority harbouring between two and four repeats. Assessment of the O-glycosylation capacity of an acceptor peptide (STPSTTTSTPSTTT), representing two of the gI blocks, showed that the peptide was a universal substrate for O-glycosylation not only for the two most commonly expressed N-acetyl-d-galactosamine (GalNAc)-T1 and -T2 transferases, but also for the GalNAc-T3, -T4 and -T11 transferases. Immunoblotting of virus-infected cells showed that gI was exclusively O-glycosylated with GalNAc monosaccharides (Tn antigen). A polymorphic mucin region has not been described previously for HSV-1 and is a unique finding, as repeated blocks within gI homologues are lacking in other alphaherpesviruses.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?