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Genotyping of clinical herpes simplex virus type 1 isolates by use of restriction enzymes.

Journal article
Authors Peter Norberg
Tomas Bergström
Jan-Åke Liljeqvist
Published in Journal of clinical microbiology
Volume 44
Issue 12
Pages 4511-4
ISSN 0095-1137
Publication year 2006
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 4511-4
Language en
Links dx.doi.org/10.1128/JCM.00421-06
Keywords Cluster Analysis, DNA Fingerprinting, methods, DNA Restriction Enzymes, DNA, Viral, genetics, Genotype, Herpes Simplex, virology, Herpesvirus 1, Human, classification, genetics, Humans, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Polymorphism, Single Nucleotide, Viral Envelope Proteins, genetics
Subject categories Medical and Health Sciences

Abstract

Recently, three distinct genotypes of clinical herpes simplex virus type 1 (HSV-1) isolates were identified based on DNA sequence information and phylogenetic analysis of clinical isolates and laboratory strains. We utilized single-nucleotide polymorphism within the genes coding for glycoproteins G and I for rapid genotype classification by PCR and restriction enzyme cleavage. The method is suitable for high-scale genotyping of clinical HSV-1 isolates and for the detection of recombinants.

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