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A method for 2D reconstruction of intracellularly labeled neurons from sequential sections.

Journal article
Authors Thomas Skoglund
Ingela Hammar
C Olsson
Claes-Henric Berthold
Published in Journal of neuroscience methods
Volume 53
Issue 2
Pages 199-202
ISSN 0165-0270
Publication year 1994
Published at Institute of Anatomy and Cell Biology
Institute of Physiology and Pharmacology
Pages 199-202
Language en
Keywords Animals, Axons, ultrastructure, Cats, Dendrites, ultrastructure, Horseradish Peroxidase, Image Processing, Computer-Assisted, instrumentation, methods, Interneurons, ultrastructure, Microscopy, Video, Neurons, ultrastructure, Software, Spinal Cord, cytology, ultrastructure
Subject categories Anatomy, Morphology, Neurophysiology


A technique for 2D reconstruction of intracellularly labeled neurons from sequential sections is described. The system consists of a Charged Coupled Device-camera mounted on a microscope, a videomixer and a IBM-compatible PC with a framegrabber. The neurons (interneurons from the spinal cord of the cat) were labeled iontophoretically by horshradish peroxidase and subsequently cut in 60 microns sections. The sections were aligned using the video mixer by fitting the cut dendrites and axon from one section with their counterparts in the following section. The images were then digitized in the PC where they were fused to create a superimposed picture of the aligned parts of the neuron; a 2D reconstruction was created.

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