To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Cholera toxin induces exp… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Cholera toxin induces expression of ion channels and carriers in rat small intestinal mucosa.

Journal article
Authors Carl-Fredrik Flach
Stefan Lange
Eva Jennische
Ivar Lönnroth
Published in FEBS letters
Volume 561
Issue 1-3
Pages 122-6
ISSN 0014-5793
Publication year 2004
Published at Institute of Medical Microbiology/Immunology
Institute of Anatomy and Cell Biology
Institute of Laboratory Medicine, Dept of Clinical Bacteriology
Pages 122-6
Language en
Links dx.doi.org/10.1016/S0014-5793(04)00...
Keywords Animals, Aquaporins, biosynthesis, Chloride Channels, biosynthesis, Cholera Toxin, pharmacology, Gene Expression Regulation, drug effects, Glucose Transporter Type 1, Intestinal Mucosa, cytology, drug effects, metabolism, Intestine, Small, cytology, Ion Channels, biosynthesis, Kinetics, Male, Membrane Transport Proteins, biosynthesis, Monosaccharide Transport Proteins, biosynthesis, Oligonucleotide Array Sequence Analysis, Rats, Rats, Sprague-Dawley
Subject categories Medical and Health Sciences

Abstract

Cholera toxin causes cyclic adenosine monophosphate (cAMP)-induced electrolyte and water secretion in the small intestine. The toxin-induced change in gene expression in rat small intestine was evaluated with microarray technique and the results were confirmed by semiquantitative polymerase chain reaction (PCR). The transporter CNT2 for nucleosides was upregulated between 6 and 18 h after challenge, whereas the level of GLUT1 transporter for glucose became elevated at 6 h. Both changes probably facilitate uptake of these nutrients in the gut. At 18 h, the major chloride channel in the villus, ClC2, was upregulated. Aquaporin 8 was downregulated at 6 h and two mucin-producing genes were upregulated 18 h after toxin challenge. The expression was back to normal after 72 h, which is the turnover time for intestinal epithelial cells.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?