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Identification of protein expression signatures associated with Helicobacter pylori infection and gastric adenocarcinoma using recombinant antibody microarrays.

Journal article
Authors Peter Ellmark
Johan Ingvarsson
Anders Carlsson
Samuel B Lundin
Christer Wingren
Carl A K Borrebaeck
Published in Molecular & cellular proteomics : MCP
Volume 5
Issue 9
Pages 1638-46
ISSN 1535-9476
Publication year 2006
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Pages 1638-46
Language en
Links dx.doi.org/10.1074/mcp.M600170-MCP2...
Keywords Adenocarcinoma, immunology, metabolism, microbiology, Aged, Aged, 80 and over, Antibodies, chemistry, Female, Helicobacter Infections, immunology, metabolism, microbiology, Helicobacter pylori, Humans, Male, Middle Aged, Protein Array Analysis, Proteomics, Recombinant Proteins, immunology, metabolism, Stomach Neoplasms, immunology, metabolism, microbiology
Subject categories Medical and Health Sciences

Abstract

Antibody microarray based technology is a powerful emerging tool in proteomics, target discovery, and differential analysis. Here, we report the first study where recombinant antibody fragments have been used to construct large scale antibody microarrays, composed of 127 different antibodies against mostly immunoregulatory antigens. The arrays were based on single framework recombinant antibody fragments (SinFabs) designed for high on-chip stability and functionality and were used for the analysis of malignant and normal stomach tissue samples from Helicobacter pylori-positive and -negative patients. Our results demonstrate that distinct tumor- as well as infection-associated protein expression signatures could be identified from these complex tissue proteomes, as well as biomarkers such as IL-9, IL-11, and MCP-4, previously not found in these diseases. In a longer perspective, this study may improve the understanding of H. pylori-induced stomach cancer and lead to development of improved diagnostics.

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