To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Mucosal immune responses … - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Mucosal immune responses are related to reduction of bacterial colonization in the stomach after therapeutic Helicobacter pylori immunization in mice

Journal article
Authors Johanna Nyström
Sukanya Raghavan
Ann-Mari Svennerholm
Published in Microbes Infect
Volume 8
Issue 2
Pages 442-9
Publication year 2006
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Pages 442-9
Language en
Keywords Animals, Antibodies, Bacterial/blood/metabolism, Bacterial Vaccines/*administration & dosage/immunology, Cholera Toxin/administration & dosage/immunology, Colony Count, Microbial, Cytokines/metabolism, Disease Models, Animal, Female, Helicobacter Infections/immunology/microbiology/*prevention & control, Helicobacter pylori/*growth & development/immunology, Humans, *Immunity, Mucosal, Immunization, Immunoglobulin A, Secretory/metabolism, Mice, Mice, Inbred C57BL, Stomach/immunology/*microbiology, T-Lymphocytes/immunology
Subject categories Medical and Health Sciences


The aim of this study was to investigate the capacity of oral and parenteral therapeutic immunization to reduce the bacterial colonization in the stomach after experimental Helicobacter pylori infection, and to evaluate whether any specific immune responses are related to such reduction. C57BL/6 mice were infected with H. pylori and thereafter immunized with H. pylori lysate either orally together with cholera toxin or intraperitoneally (i.p.) together with alum using immunization protocols that previously have provided prophylactic protection. The effect of the immunizations on H. pylori infection was determined by quantitative culture of H. pylori from the mouse stomach. Mucosal and systemic antibody responses were analyzed by ELISA in saponin extracted gastric tissue and serum, respectively, and mucosal CD4+ T cell responses by an antigen specific proliferation assay. Supernatants from the proliferating CD4+ T cells were analyzed for Th1 and Th2 cytokines. The oral, but not the parenteral therapeutic immunization induced significant decrease in H. pylori colonization compared to control infected mice. The oral immunization resulted in markedly elevated levels of serum IgG+M as well as gastric IgA antibodies against H. pylori antigen and also increased H. pylori specific mucosal CD4+ T cell proliferation with a Th1 cytokine profile. Although the parenteral immunization induced dramatic increases in H. pylori specific serum antibody titers, no increases in mucosal antibody or cellular immune responses were observed after the i.p. immunization compared to control infected mice. These findings suggest that H. pylori specific mucosal immune responses with a Th1 profile may provide therapeutic protection against H. pylori.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?