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Optical manipulation and microfluidics for studies of single cell dynamics

Review article
Authors Emma Eriksson
Jan Scrimgeour
Annette Graneli
Kerstin Ramser
Rikard Wellander
Jonas Enger
Dag Hanstorp
Mattias Goksör
Published in Journal of Optics a-Pure and Applied Optics
Volume 9
Issue 8
Pages S113-S121
ISSN 1464-4258
Publication year 2007
Published at Department of Physics (GU)
Pages S113-S121
Language en
Links dx.doi.org/10.1088/1464-4258/9/8/S0...
Subject categories Optics, Biological physics

Abstract

Most research on optical manipulation aims towards investigation and development of the system itself. In this paper we show how optical manipulation, imaging and microfluidics can be combined for investigations of single cells. Microfluidic systems have been fabricated and are used, in combination with optical tweezers, to enable environmental changes for single cells. The environment within the microfluidic system has been modelled to ensure control of the process. Three biological model systems have been studied with different combinations of optical manipulation, imaging techniques and microfluidics. In Saccharomyces cerevisiae, environmentally induced size modulations and spatial localization of proteins have been studied to elucidate various signalling pathways. In a similar manner the oxygenation cycle of single red blood cells was triggered and mapped using Raman spectroscopy. In the third experiment the forces between the endoplasmic reticulum and chloroplasts were studied in Pisum sativum and Arabidopsis thaliana. By combining different techniques we make advanced biological research possible, revealing information on a cellular level that is impossible to obtain with traditional techniques.

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