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Human serum for culture of articular chondrocytes.

Journal article
Authors Tommi Tallheden
Josefine Van Der Lee
Camilla Brantsing
Jan-Eric Månsson
Eva Sjögren-Jansson
Anders Lindahl
Published in Cell transplantation
Volume 14
Issue 7
Pages 469-79
ISSN 0963-6897
Publication year 2005
Published at Institute of Clinical Neurosciences, Section of Experimental Neuroscience
Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
Pages 469-79
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Animals, Cartilage, Articular, cytology, Cattle, Cell Culture Techniques, methods, Cell Proliferation, Chondrocytes, cytology, drug effects, Culture Media, Serum-Free, Fetus, Growth Substances, analysis, genetics, metabolism, Humans, Serum, chemistry, physiology, Tissue Engineering, methods
Subject categories Medical and Health Sciences

Abstract

In the field of cell and tissue engineering, culture expansion of human cells in monolayer plays an important part. Traditionally, cell cultures have been supplemented with serum to support attachment and proliferation, but serum is a potential source of foreign protein contamination and viral protein transmission. In this study, we evaluated the use of human serum for experimental human articular chondrocyte expansion and to develop a method for preparation of large volumes of high-quality human serum from healthy blood donors. Human autologous serum contained high levels of epidermal-derived growth factor and platelet-derived growth factor-AB and supported proliferation up to 7 times higher than FCS in primary chondrocyte cultures. By letting the coagulation take place in a commercially available transfusion bag overnight, up to 250 ml of growth factor-rich human serum could be obtained from one donor. The allogenic human serum supported high proliferation rate without losing expression of cartilage-specific genes. The expanded chondrocytes were able to redifferentiate and form cartilage matrix in comparable amounts to autologous serums. In conclusion, the transfusion bags allow preparation of large volumes of growth factor-rich human serum with the capacity to support in vitro cell expansion. The data further indicate that by controlling the coagulation process there are possibilities of optimizing the release of growth factors for other emerging cell therapies.

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