To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Epigallocatechin gallate … - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Epigallocatechin gallate increases the formation of cytosolic lipid droplets and decreases the secretion of apoB-100 VLDL

Journal article
Authors Lu Li
Pia Stillemark-Billton
Caroline Beck
Pontus Boström
Linda Andersson
Mikael Rutberg
Johanna Ericsson
Björn Magnusson
Denis Marchesan
Anna Ljungberg
Jan Borén
Sven-Olof Olofsson
Published in J Lipid Res
Volume 47
Issue 1
Pages 67-77
ISSN 0022-2275 (Print)
Publication year 2006
Published at Wallenberg Laboratory
Institute of Neuroscience and Physiology, Department of Physiology
Institute of Medicine, Department of Molecular and Clinical Medicine
Pages 67-77
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Adipocytes/drug effects/metabolism, Animals, Apolipoprotein B-100, Apolipoproteins B/genetics/*metabolism, Base Sequence, Catechin/*analogs & derivatives/pharmacology, Cell Line, Cytosol/drug effects/metabolism, DNA, Complementary/genetics, Heparin/pharmacology, Humans, Lipid Metabolism/*drug effects, Lipids/blood, Lipoproteins/blood, Lipoproteins, VLDL/*metabolism, Membrane Proteins/genetics/metabolism, Mice, Mice, Transgenic, NIH 3T3 Cells, Rats, Receptors, LDL/drug effects/metabolism, Recombinant Proteins/genetics/metabolism
Subject categories Medical and Health Sciences

Abstract

Epigallocatechin gallate (EGCG) increases the formation of cytosolic lipid droplets by a mechanism that is independent of the rate of triglyceride biosynthesis and involves an enhanced fusion between lipid droplets, a process that is crucial for their growth in size. EGCG treatment reduced the secretion of both triglycerides and apolipoprotein B-100 (apoB-100) VLDLs but not of transferrin, albumin, or total proteins, indicating that EGCG diverts triglycerides from VLDL assembly to storage in the cytosol. This is further supported by the observed increase in both intracellular degradation of apoB-100 and ubiquitination of the protein (indicative of increased proteasomal degradation) in EGCG-treated cells. EGCG did not interfere with the microsomal triglyceride transfer protein, and the effect of EGCG on the secretion of VLDLs was found to be independent of the LDL receptor. Thus, our results indicate that EGCG promotes the accumulation of triglycerides in cytosolic lipid droplets, thereby diverting lipids from the assembly of VLDL to storage in the cytosol. Our results also indicate that the accumulation of lipids in the cytosol is not always associated with increased secretion of VLDL.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?