To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Relation of the size and … - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Relation of the size and intracellular sorting of apoB to the formation of VLDL 1 and VLDL 2

Journal article
Authors Pia Stillemark-Billton
Caroline Beck
Jan Borén
Sven-Olof Olofsson
Published in J Lipid Res
Volume 46
Issue 1
Pages 104-14
ISSN 0022-2275 (Print)
Publication year 2005
Published at Wallenberg Laboratory
Pages 104-14
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Animals, Apolipoprotein B-100, Apolipoproteins B/*metabolism, Cell Line, Tumor, Immunoprecipitation, Lipoproteins, VLDL/*biosynthesis, Liver/cytology, Mice, Molecular Chaperones, Particle Size, Protein Transport, Rats, Transfection, Triglycerides/metabolism, Ultracentrifugation
Subject categories Medical and Health Sciences

Abstract

In this study, we tested the hypothesis that two separate pathways, the two-step process and an apolipoprotein B (apoB) size-dependent lipidation process, give rise to different lipoproteins. Expression of apoB-100 and C-terminally truncated forms of apoB-100 in McA-RH7777 cells demonstrated that VLDL particles can be assembled by apoB size-dependent linear lipidation, resulting in particles whose density is inversely related to the size of apoB. This lipidation results in a LDL-VLDL 2 particle containing apoB-100. VLDL 1 is assembled by the two-step process by apoB-48 and larger forms of apoB but not to any significant amount by apoB-41. The major amount of intracellular apoB-80 and apoB-100 banded with a mean density of 1.10 g/ml. Its formation was dependent on the sequence between apoB-72 and apoB-90. This dense particle, which is retained in the cell, possibly by chaperones or association with the microsomal membrane, is a precursor of secreted VLDL 1. The intracellular LDL-VLDL 2 particles formed during size-dependent lipidation appear to be the precursors of intracellular VLDL 1. We propose that the dense apoB-100 intracellular particle is converted to LDL-VLDL 2 by size-dependent lipidation. LDL-VLDL 2 is secreted or converted to VLDL 1 by the uptake of the major amount of triglycerides.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?