To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Lipopolysaccharide-induce… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Lipopolysaccharide-induced granule mobilization and priming of the neutrophil response to Helicobacter pylori peptide Hp(2-20), which activates formyl peptide receptor-like 1

Journal article
Authors Johan Bylund
Anna Karlsson
F. Boulay
Claes Dahlgren
Published in Infect Immun
Volume 70
Issue 6
Pages 2908-14
Publication year 2002
Published at Institute of Internal Medicine, Dept of Rheumatology and Inflammation Research
Pages 2908-14
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Amino Acid Sequence, Anti-Bacterial Agents/*immunology, Bacterial Proteins/*immunology, Cells, Cultured, Gelatinases/immunology, Helicobacter pylori/*immunology, Humans, Lipopolysaccharides/*immunology/pharmacology, Macrophage-1 Antigen/immunology, Molecular Sequence Data, Neutrophils/drug effects/*immunology, Organelles, Peptide Fragments/*immunology, Peptides/immunology, Receptors, Complement 3b/immunology, Receptors, Formyl Peptide, Receptors, Immunologic/*immunology, *Receptors, Lipoxin, Receptors, Peptide/*immunology, Subcellular Fractions, Superoxides
Subject categories Medical and Health Sciences

Abstract

The cecropin-like bactericidal peptide Hp(2-20) from Helicobacter pylori induces activation of the NADPH oxidase in human neutrophils via formyl peptide receptor-like 1 (FPRL1) (J. Bylund, T. Christophe, F. Boulay, T. Nystrom, A. Karlsson, and C. Dahlgren, Antimicrob. Agents Chemother. 45:1700-1704, 2001). Here we investigated the ability of bacterial lipopolysaccharide (LPS) to prime this response. Neutrophils treated with LPS for 30 min at 37 degrees C produced substantially more superoxide anion than control cells upon stimulation with Hp(2-20). Hence, LPS primed the cells for subsequent stimulation through FPRL1. To study the molecular background of this priming phenomenon, we measured the degrees of granule mobilization and concomitant receptor upregulation to the cell surface in LPS-treated cells. Exposure of complement receptors 1 and 3 as well as the formyl peptide receptor (FPR) was markedly increased after LPS treatment. Since approximately 60% of the gelatinase granules were mobilized while the specific granules were retained, we hypothesized that the gelatinase granules were potential stores of FPRL1. The presence of FPRL1 mainly in the gelatinase granules was confirmed by Western blotting of subcellular fractions of resting neutrophils. These results suggest that the mechanism behind the LPS-induced priming of FPRL1-mediated responses lies at the level of granule (receptor) mobilization.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?