To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Nuclear progesterone rece… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Nuclear progesterone receptor A and B isoforms in mouse fallopian tube and uterus: implications for expression, regulation, and cellular function

Journal article
Authors Linus Ruijin Shao
Birgitta Weijdegård
Karin Ljungström
P. Anders Friberg
Changlian Zhu
Xiaoyang Wang
Yihong Zhu
Julia Fernandez-Rodriguez
Emil Egecioglu
Emilia Rung
Håkan Billig
Published in American journal of physiology
Volume 291
Issue 1
Pages E59-72
ISSN 0193-1849 (Print)
Publication year 2006
Published at Institute of Biomedicine, Department of Medical and Clinical Genetics
Institute of Neuroscience and Physiology, Department of Physiology
Institute of Clinical Sciences
Pages E59-72
Language en
Links dx.doi.org/10.1152/ajpendo.00582.20...
Keywords Animals, Apoptosis/physiology, Blotting, Western, Chorionic Gonadotropin/physiology, Epidermal Growth Factor/antagonists & inhibitors/biosynthesis/genetics, Estradiol/blood, Estrenes/pharmacology, Fallopian Tubes/drug effects/metabolism/*physiology, Female, Furans/pharmacology, Gene Expression Regulation, Hormone Antagonists/pharmacology, Horses, Immunohistochemistry, Mice, Mice, Inbred C57BL, Mifepristone/pharmacology, Organ Size/drug effects/physiology, Progesterone/blood/pharmacology, Receptor, Epidermal Growth Factor/antagonists &, inhibitors/biosynthesis/genetics, Receptors, Progesterone/antagonists & inhibitors/biosynthesis/*physiology, Uterus/drug effects/metabolism/*physiology
Subject categories Medical and Health Sciences, Physiology

Abstract

Progesterone and its interaction with nuclear progesterone receptors (PR) PR-A and PR-B play a critical role in the regulation of female reproductive function in all mammals. However, our knowledge of the regulation and possible cellular function of PR protein isoforms in the fallopian tube and uterus in vivo is still very limited. In the present study, we revealed that equine chorionic gonadotropin (eCG) treatment resulted in a time-dependent increase in expression of both isoforms, reaching a maximal level at 48 h in the fallopian tube. Regulation of PR-A protein expression paralleled that of PR-B protein expression. However, in the uterus PR-B protein levels increased and peaked earlier than PR-A protein levels after eCG treatment. With prolonged exposure to eCG, PR-B protein levels decreased, whereas PR-A protein levels continued to increase. Furthermore, subsequent treatment with human (h)CG decreased the levels of PR protein isoforms in both tissues in parallel with increased endogenous serum progesterone levels. To further elucidate whether progesterone regulates PR protein isoforms, we demonstrated that a time-dependent treatment with progesterone (P(4)) decreased the expression of PR protein isoforms in both tissues, whereas decreases in p27, cyclin D(2), and proliferating cell nuclear antigen protein levels were observed only in the uterus. To define the potential PR-mediated effects on apoptosis, we demonstrated that the PR antagonist treatment increased the levels of PR protein isoforms, induced mitochondrial-associated apoptosis, and decreased in epidermal growth factor (EGF) and EGF receptor protein expression in both tissues. Interestingly, immunohistochemistry indicated that the induction of apoptosis by PR antagonists was predominant in the epithelium, whereas increase in PR protein expression was observed in stromal cells of both tissues. Taken together, these observations suggest that 1) the tissue-specific and hormonal regulation of PR isoform expression in mouse fallopian tube and uterus, where they are potentially involved in regulation of mitochondrial-mediated apoptosis depending on the cellular compartment; and 2) a possible interaction between functional PR protein and growth factor signaling may have a coordinated role for regulating apoptotic process in both tissues in vivo.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?