To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Epigenetic changes of ost… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Epigenetic changes of osteoblasts in response to titanium surface characteristics.

Journal article
Authors Yuki Ichioka
Farah Asa'ad
Behnosh Öhrnell Malekzadeh
Anna Westerlund
Lena Larsson
Published in Journal of biomedical materials research. Part A
ISSN 1552-4965
Publication year 2020
Published at Institute of Odontology
Institute of Odontology, Section 2
Institute of Clinical Sciences, Department of Biomaterials
Institute of Odontology, Section 3
Language en
Links dx.doi.org/10.1002/jbm.a.37014
www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords DNA damage; DNA repair; epigenetics; osteoblast; titanium.
Subject categories Dentistry, Clinical Medicine, Medical Biotechnology, Biomaterials Science

Abstract

We aimed to investigate the influence of titanium surface characteristics on epigenetic mechanisms and DNA damage/repair pathways. Osteoblast-like cells (MG63) were incubated on glass, smooth titanium, and minimally rough titanium discs, respectively, for 0, 1, 6, and 24 hr. The presence of double-stranded DNA damage (γH2AX), DNA repair (Chk2), and epigenetic markers (AcH3 & DNMT1) were investigated using immunofluorescence. There were no Chk2-positive cells on the minimally rough titanium surfaces at all-time points, in comparison to glass and smooth titanium. Total γH2AX-positive cells on minimally rough titanium gradually decreased as incubation time increased, on the contrary to smooth titanium. Minimally rough titanium surfaces induced cytoplasmic staining of DNMT1 up to 99% at 24 hr. For epigenetic markers related to the DNA damage/repair pathway, minimally rough titanium surfaces showed the lower percentage of AcH3-positive cells compared to glass and smooth titanium surface. The findings in the current study show that titanium surface characteristics indeed influence DNA damage and the DNA repair pathway, including epigenetic factors.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?