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Observation of FRET in collision of droplets

Conference paper
Authors Soumya Radhakrishnan
Felipe Ademir Alemán Hérnandez
P. T. Bhaskaran
Dag Hanstorp
Published in Optical Trapping and Optical Micromanipulation XVI. Proceedings Volume 11083. SPIE Nanoscience + Engineering, 2019, San Diego, California, United States
ISBN 978-1-5106-2860-1
Publisher SPIE
Publication year 2019
Published at Department of Physics (GU)
Language en
Links dx.doi.org/10.1117/12.2528390
Keywords Optical levitation, spectroscopy, FRET, dyes, double optical trap, collision-on-demand, coalescence
Subject categories Spectroscopy

Abstract

Forster Resonance Energy Transfer (FRET) is a radiationless distance-dependent transfer of energy from an excited donor fluorophore to an acceptor fluorophore. This radiationless interaction of a donor-acceptor pair through resonance is observed by an increase/decrease in the acceptor/donor fluorescence intensity, respectively. Here we present preliminary results on the fluorescence spectra of optically levitated micro-droplets doped with two different dyes that works as FRET pair. The laser light used for levitation (lambda=660 nm) passes through a telecentric system of lenses to form a controllable double optical trap system. Micrometer sized droplets are produced using two on-demand piezo-driven dispensers. This allows independent trapping of differently dyed droplets in two traps where a collision between the droplets can be induced by moving the trap positions. The dye molecules mix when two droplets collide and coalesce. The emission spectrum obtained when the droplets are illuminated with laser having a wavelength of 532 nm is observed with a spectrometer which can record up to 26,000 spectra per second. We compare the results with the spectra taken from the same solutions in a cuvette. The results indicate that we are able to observe the FRET effect in single droplets with an exposure time as short as 100 mu s. This spectroscopic investigation is an ongoing research project with the long-term goal to investigate environmental effects of aerosols in the atmosphere.

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