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Homogeneous Differential Magnetic Assay

Journal article
Authors S. Sepehri
T. Z. G. de la Torre
Justin F. Schneiderman
J. Blomgren
A. Jesorka
C. Johansson
M. Nilsson
J. Albert
M. Stromme
D. Winkler
A. Kalaboukhovf
Published in Acs Sensors
Volume 4
Issue 9
Pages 2381-2388
ISSN 2379-3694
Publication year 2019
Published at Institute of Neuroscience and Physiology
Pages 2381-2388
Language en
Keywords homogeneous differential magnetic assay, magnetic nanoparticle, volumetric detection, rolling circle, amplified DNA-molecules, sensitive detection, nanoparticles, diagnostics, amplification, bioassay, devices, markers, spores, Chemistry, Science & Technology - Other Topics
Subject categories Chemical Sciences


Assays are widely used for detection of various targets, including pathogens, drugs, and toxins. Homogeneous assays are promising for the realization of point-of-care diagnostics as they do not require separation, immobilization, or washing steps. For low concentrations of target molecules, the speed and sensitivity of homogeneous assays have hitherto been limited by slow binding kinetics, time-consuming amplification steps, and the presence of a high background signal. Here, we present a homogeneous differential magnetic assay that utilizes a differential magnetic readout that eliminates previous limitations of homogeneous assays. The assay uses a gradiometer sensor configuration combined with precise microfluidic sample handling. This enables simultaneous differential measurement sample containing a synthesized Vibrio cholerae target and a negative control sample, which reduces the background signal and increases the readout speed. Very low concentrations of targets down to femtomolar levels are thus detectable without any additional amplification of the number of targets. Our homogeneous differential magnetic assay method opens new possibilities for rapid and highly sensitive diagnostics at the point of care.

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