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Lipid profiling of suction blister fluid: comparison of lipids in interstitial fluid and plasma

Journal article
Authors Anders K. Nilsson
Ulrika Sjöbom
Karin Christenson
Ann Hellström
Published in Lipids in Health and Disease
Volume 18
Issue 1
ISSN 1476-511X
Publication year 2019
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Institute of Neuroscience and Physiology, Department of Clinical Neuroscience
Language en
Keywords Diacylglycerol, Highly unsaturated fatty acids, Lipidomics, Liquid chromatography-mass spectrometry, polyunsaturated fatty-acids, mass-spectrometry, omega-3, biomarkers, n-3, Biochemistry & Molecular Biology, Nutrition & Dietetics
Subject categories Nutrition and Dietetics, Biochemistry and Molecular Biology


Background Recent technical advances in the extraction of dermal interstitial fluid (ISF) have stimulated interest in using this rather unexploited biofluid as an alternative to blood for detection and prediction of disease. However, knowledge about the presence of useful biomarkers for health monitoring in ISF is still limited. In this study, we characterized the lipidome of human suction blister fluid (SBF) as a surrogate for pure ISF and compared it to that of plasma. Methods Plasma and SBF samples were obtained from 18 healthy human volunteers after an overnight fast. Total lipids were extracted and analyzed by liquid chromatography-tandem mass spectrometry. One hundred ninety-three lipid species covering 10 complex lipid classes were detected and quantified in both plasma and SBF using multiple reaction monitoring. A fraction of the lipid extract was subjected to alkaline transesterification and fatty acid methyl esters were analyzed by gas chromatography-mass spectrometry. Results The total concentration of lipids in SBF was 17% of the plasma lipid concentration. The molar fraction of lipid species within lipid classes, as well as total fatty acids, showed a generally high correlation between plasma and SBF. However, SBF had larger fractions of lysophospholipids and diglycerides relative to plasma, and consequently less diacylphospholipids and triglycerides. Principal component analysis revealed that the interindividual variation in SBF lipid profiles was considerably larger than the within-subject variation between plasma and SBF. Conclusions Plasma and SBF lipid profiles show high correlation and SBF could be used interchangeably with blood for the analysis of major lipids used in health monitoring.

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