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Strategies for Improving the Protein Yield in pH-Shift Processing of Ulva lactuca Linnaeus: Effects of Ulvan Lyases, pH-Exposure Time, and Temperature

Journal article
Authors H. Harrysson
V. R. Konasani
Gunilla B. Toth
Henrik Pavia
E. Albers
I. Undeland
Published in Acs Sustainable Chemistry & Engineering
Volume 7
Issue 15
Pages 12688-12691
ISSN 2168-0485
Publication year 2019
Published at Department of Biological and Environmental Sciences, Tjärnö Marine Biological Laboratory
Pages 12688-12691
Language en
Keywords Seaweed, Ulva lactuca, Ulvan lyase, Protein extraction, pH-shift, Protein yield, cell-wall, extraction, seaweeds, green, red, Chemistry, Science & Technology - Other Topics, Engineering, ods: implications and applications, macro and microalgae
Subject categories Biological Sciences


Globally, there is a need for novel vegetarian protein sources. We recently showed that the pH-shift process, using alkaline protein solubilization followed by isoelectric precipitation, is an efficient way to produce extracts with high protein concentrations from Ulva lactuca (>50% on a dry matter basis). However, the total protein yield was low, and to improve this, the effects of adding ulvan lyase, preincubating the seaweed homogenate at pH 8.5 and using different protein extraction temperatures (8 degrees C, RT and 40 degrees C), were evaluated in this study. Addition of ulvan lyase reduced protein solubility but increased the precipitation. Incubation at pH 8.5, without ulvan lyase added, significantly increased both protein solubility and precipitation at 8 degrees C and RT. Temperature per se had no effect on protein solubility, while protein precipitation increased with decreasing temperature. Highest protein yield (29%) was achieved when keeping the process at 8 degrees C with a preincubation step at pH 8.5 for 1 h. By these process modifications, the yield was 3.2 times higher than achieved by the control process (9.2%).

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