To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Robust Generation of Pers… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Robust Generation of Person-Specific, Synchronously Active Neuronal Networks Using Purely Isogenic Human iPSC-3D Neural Aggregate Cultures

Journal article
Authors Julia Izsak
Henrik Seth
Mats Andersson
Dzeneta Vizlin-Hodzic
S. Theiss
Eric Hanse
Hans Ågren
Keiko Funa
Sebastian Illes
Published in Frontiers in Neuroscience
Volume 13
ISSN 1662-453X
Publication year 2019
Published at Institute of Neuroscience and Physiology
Sahlgrenska Cancer Center
Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
Language en
Keywords human induced pluripotent stem cells, 3D-neural model system, neuronal networks, microelectrode, pluripotent stem-cells, in-vitro, bipolar disorder, ips cell, differentiation, abnormalities, transition, maturation, responses, ontogeny, Neurosciences & Neurology
Subject categories Neurosciences


Reproducibly generating human induced pluripotent stem cell-based functional neuronal circuits, solely obtained from single individuals, poses particular challenges to achieve personalized and patient specific functional neuronal in vitro models. A hallmark of functional neuronal assemblies, synchronous neuronal activity, can be non-invasively studied by microelectrode array (MEA) technology, reliably capturing physiological and pathophysiological aspects of human brain function. In our here presented manuscript, we demonstrate a procedure to generate 3D neural aggregates comprising astrocytes, oligodendroglial cells, and neurons obtained from the same human tissue sample. Moreover, we demonstrate the robust ability of those neurons to create a highly synchronously active neuronal network within 3 weeks in vitro, without additionally applied astrocytes. The fusion of MEA-technology with functional neuronal circuits solely obtained from one individual's cells represent isogenic person-specific human neuronal sensor chips that pave the way for specific personalized in vitro neuronal networks as well as neurological and neuropsychiatric disease modeling.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?