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Peptidomic analysis of cartilage and subchondral bone in OA patients

Journal article
Authors Birgitta Gatenholm
Johan Gobom
Tobias Skillbäck
Kaj Blennow
Henrik Zetterberg
Mats Brittberg
Published in European Journal of Clinical Investigation
Volume 49
Issue 5
ISSN 0014-2972
Publication year 2019
Published at Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
Institute of Neuroscience and Physiology, Department of Clinical Neuroscience
Institute of Clinical Sciences, Department of Orthopaedics
Language en
Keywords cartilage, mass spectrometry, neuropeptides, osteoarthritis, pain
Subject categories Orthopedics


© 2019 Stichting European Society for Clinical Investigation Journal Foundation Background: The objective of this study was to develop a method for directly analysing osteochondral samples straight out of the operating room without cell culturing, thereby enabling identification of potential peptide biomarkers to better understand the mechanisms involved in the development of osteoarthritis and pain. Material and Methods: Osteochondral plugs from wounded and macroscopically nonwounded zones of the femur condyle were collected from six patients with manifest osteoarthritis (OA) undergoing total knee arthroplasty (TKA). The samples were demineralized and supernatant was collected and isotopically marked with Tandem Mass Tag (TMT) labelling and analysed using liquid chromatography coupled with tandem mass spectrometry LC-MS/MS. Results: Using peptidomics, 6292 endogenous peptides were identified. Five hundred sixty-six peptides (8 identified endogenous peptides) differed significantly (P-value 0.10) from wounded zones compared to nonwounded zones. Conclusion: This pilot study shows promising results for enabling peptidomic analysis of cartilage and bone straight out of the operating room. With further refinement, peptidomics can potentially become a diagnostic tool for OA, and improve the knowledge of disease progression and genesis of pain.

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