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Ultrafast Glutamate Biosensor Recordings in Brain Slices Reveal Complex Single Exocytosis Transients

Journal article
Authors Yuanmo Wang
Devesh Mishra
Jenny Bergman
J. D. Keighron
Karolina P Skibicka
A. S. Cans
Published in Acs Chemical Neuroscience
Volume 10
Issue 3
Pages 1744-1752
ISSN 1948-7193
Publication year 2019
Published at Wallenberg Laboratory
Institute of Neuroscience and Physiology
Institute of Neuroscience and Physiology, Department of Physiology
Department of Chemistry and Molecular Biology
Pages 1744-1752
Language en
Links dx.doi.org/10.1021/acschemneuro.8b0...
Keywords Glutamate, biosensor, ultrafast, glutamate oxidase, gold nanoparticle, microelectrode, amperometry, exocytosis, fusion pore, dynamics, brain slice, rodent, nucleus accumbens, nucleus-accumbens, release, cells, catecholamines, events, enzyme, inputs, Biochemistry & Molecular Biology, Pharmacology & Pharmacy, Neurosciences, & Neurology, hroeder tj, 1992, analytical chemistry, v64, p3077
Subject categories Neurosciences

Abstract

Neuronal communication relies on vesicular neurotransmitter release from signaling neurons and detection of these molecules by neighboring neurons. Glutamate, the main excitatory neurotransmitter in the mammalian brain, is involved in nearly all brain functions. However, glutamate has suffered from detection schemes that lack temporal and spatial resolution allowed by electrochemistry. Here we show an amperometric, novel, ultrafast enzyme-based nanoparticle modified sensor, measuring random bursts of hundreds. to thousands of rapid spontaneous glutamate exocytotic release events at approximately 30 Hz frequency in the nucleus accumbens of rodent brain slices. Characterizing these single submillisecond exocytosis events revealed a great diversity in spike shape characteristics and size of quantal release, suggesting variability in fusion pore dynamics controlling the glutamate release by cells in this brain region. Hence, this novel biosensor allows recording of rapid single glutamate exocytosis events in the brain tissue and offers insight on regulatory aspects of exocytotic glutamate release, which is critical to understanding of brain glutamate function and dysfunction.

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