To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Mass Spectrometry Imaging… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Mass Spectrometry Imaging and Integration with Other Imaging Modalities for Greater Molecular Understanding of Biological Tissues

Review article
Authors T. P. Siegel
G. Hamm
J. Bunch
J. Cappell
John S. Fletcher
K. Schwamborn
Published in Molecular Imaging and Biology
Volume 20
Issue 6
Pages 888-901
ISSN 1536-1632
Publication year 2018
Published at Department of Chemistry and Molecular Biology
Pages 888-901
Language en
Keywords Mass spectrometry imaging (MSI), Molecular imaging, Imaging mass cytometry (IMC), Magnetic, automated anatomical interpretation, cluster ion-source, allen brain, atlas, spatial segmentation, drug distribution, maldi ims, tumor, microscopy, cells, resolution, Radiology, Nuclear Medicine & Medical Imaging, ates of america, v111, p11121, ates of america, v113, p12244
Subject categories Radiology, Nuclear Medicine and Medical Imaging, Radiological physics, Radiology


Over the last two decades, mass spectrometry imaging (MSI) has been increasingly employed to investigate the spatial distribution of a wide variety of molecules in complex biological samples. MSI has demonstrated its potential in numerous applications from drug discovery, disease state evaluation through proteomic and/or metabolomic studies. Significant technological and methodological advancements have addressed natural limitations of the techniques, i.e., increased spatial resolution, increased detection sensitivity especially for large molecules, higher throughput analysis and data management. One of the next major evolutions of MSI is linked to the introduction of imaging mass cytometry (IMC). IMC is a multiplexed method for tissue phenotyping, imaging signalling pathway or cell marker assessment, at sub-cellular resolution (1m). It uses MSI to simultaneously detect and quantify up to 30 different antibodies within a tissue section. The combination of MSI with other molecular imaging techniques can also provide highly relevant complementary information to explore new scientific fields. Traditionally, classical histology (especially haematoxylin and eosin-stained sections) is overlaid with molecular profiles obtained by MSI. Thus, MSI-based molecular histology provides a snapshot of a tissue microenvironment and enables the correlation of drugs, metabolites, lipids, peptides or proteins with histological/pathological features or tissue substructures. Recently, many examples combining MSI with other imaging modalities such as fluorescence, confocal Raman spectroscopy and MRI have emerged. For instance, brain pathophysiology has been studied using both MRI and MSI, establishing correlations between in and ex vivo molecular imaging techniques. Endogenous metabolite and small peptide modulation were evaluated depending on disease state. Here, we review advanced hot topics' in MSI development and explore the combination of MSI with established molecular imaging techniques to improve our understanding of biological and pathophysiological processes.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?