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Peripheral myeloid cells contribute to brain injury in male neonatal mice

Journal article
Authors Peter L P Smith
Amin Mottahedin
Pernilla Svedin
Carl-Johan Mohn
Henrik Hagberg
C. Joakim Ek
Carina Mallard
Published in Journal of Neuroinflammation
Volume 15
Issue 1
ISSN 1742-2094
Publication year 2018
Published at Institute of Neuroscience and Physiology, Department of Physiology
Institute of Clinical Sciences, Department of Obstetrics and Gynecology
Language en
Links dx.doi.org/10.1186/s12974-018-1344-...
Keywords Neuroinflammation, Newborn, Immune cell trafficking, central-nervous-system, developing mouse-brain, hypoxia-ischemia, neutrophil depletion, immature rat, lymphocyte trafficking, cerebral, hypoxia, temporal-changes, adult microglia, sex-differences
Subject categories Neurosciences, Physiology

Abstract

BackgroundNeonatal brain injury is increasingly understood to be linked to inflammatory processes that involve specialised CNS and peripheral immune interactions. However, the role of peripheral myeloid cells in neonatal hypoxic-ischemic (HI) brain injury remains to be fully investigated.MethodsWe employed the Lys-EGFP-ki mouse that allows enhanced green fluorescent protein (EGFP)-positive mature myeloid cells of peripheral origin to be easily identified in the CNS. Using both flow cytometry and confocal microscopy, we investigated the accumulation of total EGFP(+) myeloid cells and myeloid cell subtypes: inflammatory monocytes, resident monocytes and granulocytes, in the CNS for several weeks following induction of cerebral HI in postnatal day 9 mice. We used antibody treatment to curb brain infiltration of myeloid cells and subsequently evaluated HI-induced brain injury.ResultsWe demonstrate a temporally biphasic pattern of inflammatory monocyte and granulocyte infiltration, characterised by peak infiltration at 1day and 7days after hypoxia-ischemia. This occurs against a backdrop of continuous low-level resident monocyte infiltration. Antibody-mediated depletion of circulating myeloid cells reduced immune cell accumulation in the brain and reduced neuronal loss in male but not female mice.ConclusionThis study offers new insight into sex-dependent central-peripheral immune communication following neonatal brain injury and merits renewed interest in the roles of granulocytes and monocytes in lesion development.

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