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Glycosphingolipids of porcine, bovine, and equine pericardia as potential immune targets in bioprosthetic heart valve grafts

Journal article
Authors Angela Barone
John Benktander
Christy Whiddon
Chunsheng Jin
C. Galli
Susann Teneberg
Michael Breimer
Published in Xenotransplantation
Volume 25
Issue 5
ISSN 0908-665X
Publication year 2018
Published at Institute of Clinical Sciences, Department of Surgery
Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Language en
Links dx.doi.org/10.1111/xen.12406
Keywords bioprosthetic heart valves, carbohydrate antigens, glycosphingolipid characterization, immune, blood-group-a, alpha-gal, nonacid glycosphingolipids, human, erythrocytes, gangliosides, expression, implantation, spectrometry, galactose, kidney, Research & Experimental Medicine, Transplantation
Subject categories Clinical Medicine

Abstract

BackgroundPericardial tissue from various animal species is utilized for the production of the bioprosthetic heart valves (BHV) used clinically. Experimental data show that the eventual breakdown of BHV is partly due to immunological interactions with carbohydrate tissue antigens. To understand these processes, we have examined the glycolipid-based carbohydrate antigens in naive porcine, bovine, and equine pericardia. ExperimentalTotal non-acid and acid glycosphingolipid fractions were isolated from porcine, bovine, and equine pericardia, and individual glycolipid compounds were characterized by thin-layer chromatography, mass spectrometry, and binding of monoclonal antibodies, lectins and bacteria in chromatogram binding assays. ResultsThe non-acid glycolipid fractions from all species contained glycosphingolipids based on the globo- and neolacto-series, including pentaglycosylceramides with terminal Gal3 determinants. Terminal blood group A and H (O) structures based on type 2 core chains were present in porcine pericardium, while the Forssman pentaosylceramide was found in equine pericardium. All acid glycolipid fractions contained sulfatide and several gangliosides with both N-acetyl- and N-glycolyl-neuraminic acid as terminal saccharide chain determinants. ConclusionSeveral carbohydrate antigens which are potential targets for the human immune system have been identified in the animal pericardial tissues used for the production of BHV. Which of these antigens are left in the tissues after industrial BHV production processes, as well as their potential role in eventual BHV degradation, remains to be elucidated.

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