To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Intestinal and circulatin… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Intestinal and circulating antibody-forming cells in IgA-deficient individuals after oral cholera vaccination.

Journal article
Authors Vanda Friman
Marianne Quiding-Järbrink
L Larsson
D Ericson
J Björkander
K Theman
Jan Holmgren
Published in Clinical and experimental immunology
Volume 95
Issue 2
Pages 222-6
ISSN 0009-9104
Publication year 1994
Published at Institute of Internal Medicine, Dept of Infectious Diseases
Institute of Medical Microbiology/Immunology
Pages 222-6
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Administration, Oral, Adult, Aged, Antibody-Producing Cells, physiology, Cholera Vaccines, administration & dosage, immunology, Duodenum, immunology, Female, Humans, IgA Deficiency, immunology, Immunoglobulin M, analysis, Intestinal Mucosa, immunology, Male, Middle Aged, Vaccination
Subject categories Infectious Medicine

Abstract

In search for a possible explanation for the different susceptibility to mucosal infections in IgA-deficient (IgAd) individuals, the frequency of total immunoglobulin-secreting cells (ISC) and vaccine-specific antibody-secreting cells (ASC) in intestinal mucosa and peripheral blood was determined by the enzyme-linked immunospot (ELISPOT) assay before and after peroral vaccination with a B subunit-whole cell cholera vaccine. Two groups of IgAd individuals, frequently infected and non-infected respectively, and normal controls were studied. Before cholera vaccination there were significantly higher frequencies of total IgM and IgG ISC in the gut, but not in the blood, in the IgAd individuals than in the controls. However, there were no significant differences between healthy and infection-prone IgAd individuals in this respect. In response to oral cholera vaccination, intestinal cholera toxin (CT)-specific IgG and IgM ASC were significantly more abundant among the IgAd individuals with a history of frequent infections than among the healthy IgAd individuals and controls. A similar difference in IgG and IgM ASC, although not significant, was also noted in blood. In IgAd individuals with frequent infections the vaccine induced variable anti-CT IgM ASC responses in the gut, ranging from no increase to a few strikingly high responses. In the controls, the CT-specific responses were dominated by IgA ASC. The data show that oral cholera vaccination evoked strong CT-specific IgG ASC responses, and in some cases also strong IgM ASC responses in the intestinal mucosa of IgAd patients with a history of frequent infections. The healthy IgAd individuals unexpectedly responded with lower numbers of CT-specific IgG ASC and did not show any increase of CT-specific IgM ASC in the intestinal mucosa. Thus, inability to mount a mucosal immune response to an oral antigen cannot in itself explain recurrent infections among many IgAd individuals.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?