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Growth of a common planktonic diatom quantified using solid medium culturing.

Journal article
Authors Olga Kourtchenko
Tuomas Rajala
Anna Godhe
Published in Scientific reports
Volume 8
Issue 1
ISSN 2045-2322
Publication year 2018
Published at Department of Mathematical Sciences
Department of marine sciences
Language en
Links dx.doi.org/10.1038/s41598-018-28129...
www.ncbi.nlm.nih.gov/entrez/query.f...
Subject categories Ecology, Ocean and River Engineering, Evolutionary Biology, Microbiology

Abstract

The ability to grow on solid culture medium is a pre-requisite for a successful microbial genetic model organism. Skeletonema marinoi, a bloom-forming, planktonic marine microalga, is widely used in ecological, evolutionary and population genetics studies. We have tested and confirmed the ability of this common organism to grow on solid culture medium (agar) under experimentally manipulated conditions. We established a protocol for quantifying growth characteristics - length of lag phase, growth rate, maximum biomass yield - on agar medium. The procedure was tested under experimental treatments and the resulting growth changes correlated with those observed in standard liquid culture. The ability to grow on solid medium broadens the use of S. marinoi as a molecular model, where agar is routinely used for various purposes (growth, selection, storage); and the possibility to quantify colony growth opens the way for high throughput, automated, or semi-automated phenotyping solutions.

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