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3D ultrastructure of multi-vesicular bodies in fission yeast

Journal article
Authors Davide Zabeo
Rossella Crescitelli
Eileen O'Toole
Helio Roque
Johanna L Höög
Published in Science Matters
Volume 3
Issue 3
ISSN 2297-8240
Publication year 2017
Published at Krefting Research Centre
Department of Chemistry and Molecular Biology
Language en
Links https://doi.org/10.19185/matters.20...
https://www.sciencematters.io/artic...
Subject categories Microbiology, Cell and Molecular Biology, Cell Biology, Biological Sciences

Abstract

The multi-vesicular body (MVB) organelle and the cellular sorting pathway associated with it are very well characterized in budding yeast (Saccharomyces cerevisiae). However, little is known about MVB structure and function in fission yeast (Schizosaccharomyces pombe). In this work, we investigated and characterized the three-dimensional ultrastructure of MVBs in S. pombe using electron tomography. We discovered a positive correlation between MVB size and the number of intralumenal vesicles (ILVs) contained in them. MVBs grew larger with the progression of the cell cycle, hinting at an unknown interaction between the regulation of the two processes. Larger MVBs were also observed in the temperature-sensitive cdc25-22 mutant, which is defective in cell cycle progression into mitosis at the restrictive temperature. This study offers, to our knowledge, the first electron microscopic observation and tomographic reconstruction of MVBs in S. pombe.

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