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Spatially confined photoinactivation of bacteria: Towards novel tools for detailed mechanistic studies

Conference paper
Authors Hanna Thomsen
Jeemol James
Anne Farewell
Marica B Ericson
Published in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Multiphoton Microscopy in the Biomedical Sciences XVIII 2018; San Francisco; United States; 28 January 2018 through 30 January 2018
ISBN 978-1-5106-1482-6
ISSN 1605-7422
Publication year 2018
Published at Department of Chemistry and Molecular Biology
Centre for antibiotic resistance research, CARe
Language en
Keywords 3D, Antimicrobial resistance, biofilms, staphylococcus epidermidis, two photon microscopy
Subject categories Microbiology, Biophysics, Spectroscopy


© 2018 SPIE. Antimicrobial resistance is a serious global threat fueling an accelerated field of research aimed at developing novel antimicrobial therapies. A particular challenge is the treatment of microbial biofilms formed upon bacterial growth and often associated with chronic infections. Biofilms comprise bacteria that have adhered to a surface and formed 3D microcolonies, and demonstrate significantly increased antimicrobial resistance compared to the planktonic counterpart. A challenge in developing novel strategies for fighting these chronic infections is a lack of mechanistic understanding of what primarily contributes to enhanced drug resistance. Tools for noninvasive study of live biofilms are necessary to begin to understand these mechanisms on both a single cell and 3D level. Herein, a method by which multiphoton microscopy is implemented to study a biofilm model of Staphylococcus epidermidis to noninvasively visualize and measure penetration of compounds in 3D biofilm structure and two photon excitation was exploited for spatially confined photoinactivation and microscopy optimized for evaluation of microbiological viability at a microscopic level. Future studies are aimed at future development of the proposed techniques for detailed studies of, e.g., quorum sensing and mechanisms contributing to antimicrobial resistance.

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