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Applying microfluidic systems to study effects of glucose at single-cell level

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Authors Niek Welkenhuysen
Caroline B. Adiels
Mattias Goksör
Stefan Hohmann
Published in Glucose Transport. Methods in Molecular Biology
ISBN 978-1-4939-7507-5
Publisher Humana Press
Place of publication New York
Publication year 2018
Published at Department of Chemistry and Molecular Biology
Department of Physics (GU)
Language en
Links dx.doi.org/10.1007/978-1-4939-7507-...
Keywords Fluorescence microscopy, Glucose uptake, Microbiology, Microfluidic system, Optical tweezers, Saccharomyces cerevisiae, Single cell analysis, glucose, microfluidics, nonhuman
Subject categories Physical Sciences

Abstract

Microfluidic systems in combination with microscopy (e.g., fluorescence) can be a powerful tool to study, at single-cell level, the behavior and morphology of biological cells after uptake of glucose. Here, we briefly discuss the advantages of using microfluidic systems. We further describe how microfluidic systems are fabricated and how they are utilized. Finally, we discuss how the large amount of data can be analyzed in a “semi-automatic” manner using custom-made software. In summary, we provide a guide to how to use microfluidic systems in single-cell studies. © Springer Science+Business Media LLC 2018.

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