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Raman imaging of floating cells

Journal article
Authors Caitriona M. Creely
Giovanni Volpe
Gajendra P. Singh
Marta Soler
Dmitri V. Petrov
Published in Optics Express
Volume 13
Pages 6105-6110
ISSN 1094-4087
Publication year 2005
Published at
Pages 6105-6110
Language en
Subject categories Cell Biology, Other Physics Topics

Abstract

Raman imaging can yield spatially resolved biochemical information from living cells. To date there have been no Raman images published of cells in suspension because of the problem of immobilising them suitably to acquire space-resolved spectra. In this paper in order to overcome this problem the use of holographic optical tweezers is proposed and implemented, and data is shown for spatially resolved Raman spectroscopy of a live cell in suspension. © 2005 Optical Society of America.

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