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The pht4;1-3 mutant line contains a loss of function allele in the fatty acid desaturase 7 gene caused by a remnant inactivated selection marker- a cautionary tale

Journal article
Authors Anders K. Nilsson
Mats X. Andersson
Published in PeerJ
Volume 5
ISSN 2167-8359
Publication year 2017
Published at Department of Biological and Environmental Sciences
Language en
Links dx.doi.org/10.7717/peerj.4134
Keywords Arabidopsis insertion mutant, FAD7, PHT4, Transposon mutant, Trienoic fatty acids
Subject categories Genetics, Botany

Abstract

© 2017 Nilsson and Andersson. Astriking and unexpected biochemical phenotype was found in an insertion mutant line in the model plant Arabidopsis thaliana. One of two investigated insertion mutant lines in the gene encoding the phosphate transporter PHT4;1 demonstrated a prominent loss of trienoic fatty acids, whereas the other insertion line was indistinguishable from wild type in this aspect. We demonstrate that the loss of trienoic fatty acids was due to a remnant inactive negative selection marker gene in this particular transposon tagged line, pht4;1-3. This constitutes a cautionary tale that warns of the importance to confirm the loss of this type of selection markers and the importance of verifying the relationship between a phenotype and genotype by more than one independent mutant line or alternatively genetic complementation.

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