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Optimization of a high-throughput phenotyping method for chain-forming phytoplankton species

Journal article
Authors Susanna Gross
Olga Kourtchenko
Tuomas Rajala
Björn Andersson
Luciano Francisco Fernandez Ricaud
Anders Blomberg
Anna Godhe
Published in Limnology and Oceanography : Methods
Volume 16
Issue 2
Pages 57-67
ISSN 1541-5856
Publication year 2018
Published at Department of Mathematical Sciences
Department of marine sciences
Department of Chemistry and Molecular Biology
Pages 57-67
Language en
Links dx.doi.org/10.1002/lom3.10226
onlinelibrary.wiley.com/wol1/doi/10...
Subject categories Microbiology, Ecology

Abstract

Modern equipment facilitates phenotyping of hundreds of strains of unicellular organisms by culturing and monitoring growth in microplates. However, in the field of phytoplankton ecology, automated monitoring of growth is not often done and this method has not been tested for many species. To meet the demand for a high-throughput technique for monitoring growth of chain-forming phytoplankton species, we have assessed and optimized a method commonly used for other microorganisms. Skeletonema marinoi is a pelagic chain-forming diatom, and we have acquired growth patterns in four different treatments (i.e., low and high light, low and high nutrient concentrations) when cultured in multi-well plates. Due to the unexpected heterogeneity in growth rates and maximum cell densities observed between wells (spatial) and runs (temporal), a set of models was fitted to the obtained phenotypic data to correct for these biases. Models were tested for robustness on two replicate multi-strain experiments including 23 different strains. Using the model accounting for temporal and spatial bias, we could reliably determine changes in growth rate caused by nutrient treatments as well as differences in cell density as a response to nutrient availability and light treatment. This method can facilitate high-throughput phenotyping of hundreds of strains, which is often a bottleneck in characterizing the ecology and capacity for adaptation of chain-forming phytoplankton.

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