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Identification and characterization of the novel colonization factor CS30 based on whole genome sequencing in enterotoxigenic Escherichia coli (ETEC)

Journal article
Authors Astrid von Mentzer
Joshua Tobias
Gudrun Wiklund
Stefan Nordqvist
M. Aslett
G. Dougan
A. Sjoling
Ann-Mari Svennerholm
Published in Scientific Reports
Volume 7
ISSN 2045-2322
Publication year 2017
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Language en
Links doi.org/10.1038/s41598-017-12743-3
Keywords phase variation, type-1 fimbriae, regulatory gene, incf plasmids, expression, diarrhea, antigen, adhesin, vaccine, epidemiology, Science & Technology - Other Topics
Subject categories Epidemiology

Abstract

The ability to colonize the small intestine is essential for enterotoxigenic Escherichia coli (ETEC) to cause diarrhea. Although 22 antigenically different colonization factors (CFs) have been identified and characterized in ETEC at least 30% of clinical ETEC isolates lack known CFs. Ninety-four whole genome sequenced "CF negative" isolates were searched for novel CFs using a reverse genetics approach followed by phenotypic analyses. We identified a novel CF, CS30, encoded by a set of seven genes, csmA-G, related to the human CF operon CS18 and the porcine CF operon 987P (F6). CS30 was shown to be thermo-regulated, expressed at 37 degrees C, but not at 20 degrees C, by SDS-page and mass spectrometry analyses as well as electron microscopy imaging. Bacteria expressing CS30 were also shown to bind to differentiated human intestinal Caco-2 cells. The genes encoding CS30 were located on a plasmid (E873p3) together with the genes encoding LT and STp. PCR screening of ETEC isolates revealed that 8.6% (n = 13) of "CF negative" (n = 152) and 19.4% (n = 13) of " CF negative" LT + STp (n = 67) expressing isolates analyzed harbored CS30. Hence, we conclude that CS30 is common among " CF negative" LT + STp isolates and is associated with ETEC that cause diarrhea. RAHAM JM, 1985, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES

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