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Reactivation of Gαi-coupled formyl peptide receptors is inhibited by Gαq-selective inhibitors when induced by signals generated by the platelet-activating factor receptor.

Journal article
Authors André Holdfeldt
Agnes Dahlstrand Rudin
Michael Gabl
Zahra Rajabkhani
Gabriele M König
Evi Kostenis
Claes Dahlgren
Huamei Forsman
Published in Journal of Leukocyte Biology
Volume 102
Issue 3
Pages 871-880
ISSN 0741-5400
Publication year 2017
Published at Institute of Odontology
Institute of Medicine, Department of Rheumatology and Inflammation Research
Pages 871-880
Language en
Links dx.doi.org/10.1189/jlb.2A0317-086RR
www.ncbi.nlm.nih.gov/entrez/query.f...
Subject categories Cell and Molecular Biology

Abstract

Formyl peptide receptor (FPR)-desensitized neutrophils display increased production/release of superoxide (O2(-)) when activated by platelet-activating factor (PAF), a priming of the response achieved through a unique receptor crosstalk mechanism. The aim of this study was to determine the effect of an inhibitor selective for small, heterotrimeric G proteins belonging to the Gαq subclass on that receptor crosstalk. We show that signals generated by FPRs and the PAF receptor (PAFR) induce activation of the neutrophil O2(-), producing NADPH-oxidase, and that response was sensitive to Gαq inhibition in cells activated by PAF, but no inhibition was obtained in cells activated by FPR agonists. Signaling in naive neutrophils is terminated fairly rapidly, and the receptors become homologously desensitized. The downstream sensitivity to Gαq inhibition in desensitized cells displaying increased production/release of O2(-) through the PAFR receptor crosstalk mechanism also comprised the reactivation of the FPRs, and the activation signals were redirected from the PAFR to the desensitized/reactivated FPRs. The Gαq-dependent activation signals generated by the PAFRs activate the Gαi-coupled FPRs, a receptor crosstalk that represents a novel pathway by which G protein-coupled receptors can be regulated and signaling can be turned on and off.

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