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A sequential assessment of the preservation injury in porcine intestines

Journal article
Authors Mihai Oltean
L. Jiga
Mats Hellström
John M. Söfteland
M. Papurica
T. Hoinoiu
M. Ionac
Anna Casselbrant
Published in Journal of Surgical Research
Volume 216
Pages 149-157
ISSN 0022-4804
Publication year 2017
Published at Institute of Clinical Sciences, Department of Surgery
Institute of Clinical Sciences, Department of Obstetrics and Gynecology
Pages 149-157
Language en
Links doi.org/10.1016/j.jss.2017.05.002
Keywords small-bowel preservation, ischemia-reperfusion injury, of-wisconsin, solution, tight junction, cold preservation, mucosal lesion, transplantation, rat, expression, apoptosis, Surgery, iu cj, 1970, archives of surgery, v101, p478
Subject categories Surgery

Abstract

Background: Clinical and experimental evidence strongly suggest that ischemia-reperfusion injury after intestinal transplantation has deleterious short-and long-term effects and finding means to reduce ischemia-reperfusion injury is a major research area. The anatomical and physiological similarities between the human and porcine digestive tract favor its use as a preclinical model for translational research. Intriguingly, no systematic appraisal of the development of the intestinal preservation injury in pigs is available. Materials and methods: Intestinal procurement was performed in nine pigs using histidine-tryptophan-ketoglutarate solution as preservation fluid. Ileal biopsies were obtained after 8, 14, and 24 h of static cold storage (SCS), and the preservation injury was assessed morphologically (Chiu score) as well as on the molecular level. Tight junction (zonula occludens, claudin-3 and 4, tricellulin, and occludin) and adherens junctions (E-cadherin) proteins were studied using immunofluorescence and Western blot. Results: Eight hours of SCS induced minimal mucosal changes (Chiu grade 1) that advanced to significant subepithelial edema (Chiu grade 3) after 24 h; progressive Goblet cell depletion was also noted. Apoptosis (studied by cleaved caspase-3 staining significantly increased after 24 h of SCS. Significant molecular changes with decreasing expression of zonula occludens, tricellulin, and occludin were evident already after 8 h of SCS and continuously worsened. Claudin-3 and Claudin-4 and E-cadherin expression remained relatively unaltered during SCS. Conclusions: Important molecular alterations precede histologic changes during SCS of the porcine intestine and may be used as more sensitive injury markers than histologic changes in intestinal ischemia and transplantation. (C) 2017 Elsevier Inc. All rights reserved.

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