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Time-kill kinetic analysis of antimicrobial chemotherapy based on hydrogen peroxide photolysis against Streptococcus mutans biofilm

Journal article
Authors M. Shirato
K. Nakamura
T. Kanno
Peter Lingström
Y. Niwano
Ulf Örtengren
Published in Journal of Photochemistry and Photobiology B-Biology
Volume 173
Pages 434-440
ISSN 1011-1344
Publication year 2017
Published at Institute of Odontology, Section 3
Pages 434-440
Language en
Keywords Biofilm, Chlorhexidine gluconate, Hydrogen peroxide, Hydroxyl radical, Photodynamic therapy, Streptococcus mutans, photodynamic therapy, dental-caries, chlorhexidine varnish, bactericidal, action, toluidine blue, in-vitro, photosensitizers, disinfection, children, agents, Biochemistry & Molecular Biology, Biophysics
Subject categories Dentistry


A recently developed antimicrobial technique utilizing hydroxyl radicals generated by hydrogen peroxide (H2O2) photolysis represents a promising new therapy for preventing and treating dental caries. The present study compared the antimicrobial time-kill kinetics of H2O2 photolysis, conventional antiseptics, and antimicrobial photodynamic therapy (aPDT) against biofilm-forming Streptococcus mutans (cariogenic bacteria) grown on hydroxyapatite disks. H2O2 photolysis was performed by irradiating the biofilm immersed in 3% H2O2 with 365-nm light-emitting diode (LED) light at an irradiance of 1000 mW/cm(2) for up to 1.5 min. Antiseptic treatments consisted of 0.2% chlorhexidine gluconate, 0.5% povidone-iodine, and 3% H2O2. The biofilm was immersed in each antiseptic for up to 4 min. aPDT was performed by irradiating the biofilm immersed in 100 Oil methylene blue or toluidine blue O with 655-nm laser light at 1000 mW/cm(2) for up to 4 min. Based on the time kill assay, the decimal reduction value (D-value) of each treatment was determined. With a D-value of 0.06 min, H2O2 photolysis exhibited the highest bactericidal effect against biofilm-forming S. mutans. In contrast, antiseptics and aPDT exerted a slower bactericidal effect, with D-values of 0.9-2.7 min. In conclusion, the antimicrobial technique based on H2O2 photolysis using 365-nm LED represents a strong adjunctive chemotherapy for dental caries treatment.

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