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NGS-based biodiversity and community structure analysis of meiofaunal eukaryotes in shell sand from Hållö island, Smögen, and soft mud from Gullmarn Fjord, Sweden

Journal article
Authors Quiterie Haenel
Oleksandr Holovachov
Ulf Jondelius
Per Sundberg
Sarah Bourlat
Published in Biodiversity Data Journal
Volume 5
Publication year 2017
Published at Department of marine sciences
Language en
Links https://doi.org/10.3897/BDJ.5.e1273...
Keywords 18S, COI, Community structure, Illumina Mi-Seq, Meiofaunal biodiversity, Metabarcoding
Subject categories Genetics, Zoology, Biological Systematics, Bioinformatics and Systems Biology


© Haenel Q et al. Aim: The aim of this study was to assess the biodiversity and community structure of Swedish meiofaunal eukaryotes using metabarcoding. To validate the reliability of the metabarcoding approach, we compare the taxonomic resolution obtained using the mitochondrial cytochrome oxidase 1 (COI) 'mini-barcode' and nuclear 18S small ribosomal subunit (18S) V1-V2 region, with traditional morphology-based identification of Xenacoelomorpha and Nematoda. Location: 30 samples were analysed from two ecologically distinct locations along the west coast of Sweden. 18 replicate samples of coarse shell sand were collected along the northeastern side of Hållö island near Smögen, while 12 replicate samples of soft mud were collected in the Gullmarn Fjord near Lysekil.

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