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Activation of complement factor B contributes to murine and human myocardial ischemia/reperfusion injury

Journal article
Authors N. Chun
A. S. Haddadin
J. Y. Liu
Y. F. Hou
K. A. Wong
D. Lee
J. I. Rushbrook
K. Gulaya
R. Hines
T. Hollis
B. N. Nuno
A. A. Mangi
S. Hashim
Marcela Pekna
A. Catalfamo
H. Y. Chin
F. Patel
S. Rayala
K. Shevde
P. S. Heeger
M. Zhang
Published in PLoS ONE
Volume 12
Issue 6
ISSN 1932-6203
Publication year 2017
Published at Institute of Neuroscience and Physiology, Department of Clinical Neuroscience
Language en
Links doi.org/10.1371/journal.pone.017945...
Keywords ischemia-reperfusion injury, mannose-binding lectin, bypass, graft-surgery, open-heart-surgery, percutaneous coronary intervention, alternative pathway complement, cardiac troponin-i, c-reactive protein, nf-kappa-b, cardiopulmonary bypass, Science & Technology - Other Topics
Subject categories Cardiac and Cardiovascular Systems

Abstract

The pathophysiology of myocardial injury that results from cardiac ischemia and reperfusion (I/R) is incompletely understood. Experimental evidence from murine models indicates that innate immune mechanisms including complement activation via the classical and lectin pathways are crucial. Whether factor B (f B), a component of the alternative complement pathway required for amplification of complement cascade activation, participates in the pathophysiology of myocardial I/R injury has not been addressed. We induced regional myocardial I/R injury by transient coronary ligation in WT C57BL/6 mice, a manipulation that resulted in marked myocardial necrosis associated with activation of fB protein and myocardial deposition of C3 activation products. In contrast, in f13(-/-) mice, the same procedure resulted in significantly reduced myocardial necrosis (% ventricular tissue necrotic; fB(-/-) mice, 20 4%; WT mice, 45 3%; P< 0.05) and diminished deposition of C3 activation products in the myocardial tissue (fB(-/-) mice, 0 0%; WT mice, 31 6%; P<0.05). Reconstitution of fB(-/-) mice with WT serum followed by cardiac I/R restored the myocardial necrosis and activated C3 deposition in the myocardium. In translational human studies we measured levels of activated fB (Bb) in intracoronary blood samples obtained during cardio-pulmonary bypass surgery before and after aortic cross clamping (AXCL), during which global heart ischemia was induced. Intracoronary Bb increased immediately after AXCL, and the levels were directly correlated with peripheral blood levels of cardiac troponin I, an established biomarker of myocardial necrosis (Spearman coefficient = 0.465, P < 0.01). Taken together, our results support the conclusion that circulating fB is a crucial pathophysiological

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