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Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections

Journal article
Authors M. C. Zhao
G. X. Li
D. Zhang
H. Y. Zhou
Hao Wang
S. Yang
L. Wang
Z. S. Feng
X. J. Ma
Published in Diagnostic Microbiology and Infectious Disease
Volume 88
Issue 2
Pages 115-119
ISSN 0732-8893
Publication year 2017
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 115-119
Language en
Keywords Evaluation, Mycoplasma pneumoniae, Chlamydia, Respiratory virus, Co-infection, Multiplex reverse, TRACHOMATIS, SPECIMENS, DIAGNOSIS, SYSTEM, CHINA, PANEL
Subject categories Microbiology in the medical area, Infectious Medicine


Respiratory Pathogen 13 Detect:ion Kit (13x kit) is able to simultaneously detect 11 respiratory viruses, Mycoplasma pneumoniae (MP) and Chlamydia in a single reaction. Using 572 Nasopharyngeal aspirates collected from hospitalized children, the clinical performance of 13x kit for detecting 11 respiratory viruses was evaluated in comparison with a routinely used 2-tube multiplex reverse transcription PCR assay (2-tube assay) at provincial Centers for Disease Control and Prevention in China. The clinical performance of 13x kit for detecting MP and Chlamydia was evaluated by commercial real-time quantitative PCR (qPCR) kits or sequencing. For tested viruses, the assay concordance was 95.98% and the kappa coefficient was 0.89. All the MP and Chlamydia positive samples detected by 13x kit were confirmed as true positives. The utilization of the 13x kit in clinical settings will be helpful for doctors to assess clinical outcome according to virus type or multiple infections, and to limit the use of antibiotics. (C) 2017 Elsevier Inc. All rights reserved.

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