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Molecular Insights into Covalently Stained Carious Dentine Using Solid-State NMR and ToF-SIMS

Journal article
Authors Ulrica S. Almhöjd
Peter Lingström
Åke Nilsson
Jörgen G Norén
S. Siljestrom
A. Ostlund
D. Bernin
Published in Caries Research
Volume 51
Issue 3
Pages 255-263
ISSN 0008-6568
Publication year 2017
Published at Institute of Odontology
Department of Chemistry and Molecular Biology
Institute of Odontology, Section 3
Pages 255-263
Language en
Links https://doi.org/10.1159/000460828
Keywords Carious dentine, Covalent binding, Hydrazine, Normal dentine, NMR, ToF-SIMS, matrix proteins, peritubular dentin, cavity preparation, in-vitro, bone, c-13, spectroscopy, collagen, n-15, dye, Dentistry, Oral Surgery & Medicine
Subject categories Surgical research, Dentistry

Abstract

Dyes currently used to stain carious dentine have a limited capacity to discriminate normal dentine from carious dentine, which may result in overexcavation. Consequently, finding a selective dye is still a challenge. However, there is evidence that hydrazine-based dyes, via covalent bonds to functional groups, bind specifically to carious dentine. The aim of this study was to investigate the possible formation of covalent bonds between carious dentine and N-15(2)-hydrazine and the hydrazine-based dye, N-15(2)-labelled Lucifer Yellow, respectively. Powdered dentine from extracted carious and normal teeth was exposed to the dyes, and the staining reactions were analysed using time-of-flight secondary ion mass spectrometry (ToF-SIMS), solid-state C-13-labelled nuclear magnetic resonance (NMR) and N-15-NMR spectroscopy. The results showed that N-15(2)-hydrazine and N-15(2)-labelled Lucifer Yellow both bind to carious dentine but not to normal dentine. It can thus be concluded that hydrazine-based dyes can be used to stain carious dentine and leave normal dentine unstained. (C) 2017 S. Karger AG, Basel

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