To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Hypothermia after cranial… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Hypothermia after cranial irradiation protects neural progenitor cells in the subventricular zone but not in the hippocampus.

Journal article
Authors Magnus Sabel
Marie Kalm
Thomas Björk-Eriksson
Birgitta Lannering
Klas Blomgren
Published in International journal of radiation biology
Volume 93
Issue 8
Pages 771-783
ISSN 1362-3095
Publication year 2017
Published at Institute of Neuroscience and Physiology
Institute of Clinical Sciences, Department of Oncology
Institute of Clinical Sciences, Department of Pediatrics
Pages 771-783
Language en
Links dx.doi.org/10.1080/09553002.2017.13...
www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Iba1; Radiotherapy; brain; granule cell layer; neurogenesis; phospho-histone H3
Subject categories Neurobiology, Pediatrics, Cancer and Oncology

Abstract

To explore if hypothermia can reduce the harmful effects of ionizing radiation on the neurogenic regions of the brain in young rats.Postnatal day 9 rats were randomized into two treatment groups, hypo- and normothermia, or a control group. Treatment groups were placed in chambers submerged in temperature-controlled water baths (30˚C and 36˚C) for 8 h, after receiving a single fraction of 8 Gy to the left hemisphere. Seven days post-irradiation, we measured the sizes of the subventricular zone (SVZ) and the granule cell layer (GCL) of the hippocampus, and counted the number of proliferating (phospho-histone H3+) cells and microglia (Iba1+ cells).Irradiation caused a 53% reduction in SVZ size in the normothermia group compared to controls, as well as a reduction of proliferating cell numbers by >50%. These effects were abrogated in the hypothermia group. Irradiation reduced the number of microglia in both treatment groups, but resulted in a lower cell density of Iba1+ cells in the SVZs of the hypothermia group. In the GCL, irradiation decreased both GCL size and the proliferating cell numbers, but with no difference between the treatment groups. The number of microglia in the GCL did not change.Hypothermia immediately after irradiation protects the SVZ and its proliferative cell population but the GCL is not protected, one week post-irradiation.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?