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Surface characterization of insulin-coated Ti6Al4V medical implants conditioned in cell culture medium: An XPS study

Journal article
Authors Andrey Shchukarev
Behnosh Öhrnell Malekzadeh
Maria Ransjö
Pentti Tengvall
Anna Westerlund
Published in Journal of Electron Spectroscopy and Related Phenomena
Volume 216
Pages 33-38
ISSN 0368-2048
Publication year 2017
Published at Institute of Odontology
Institute of Clinical Sciences, Department of Biomaterials
Institute of Odontology, Section 3
Pages 33-38
Language en
Keywords Cell culture medium, Insulin, Proteins at interface, Titanium implant, XPS
Subject categories Medical Biotechnology, Biomaterials Science


© 2017 Elsevier B.V.Surface characterization of insulin-coated Ti6Al4V medical implants, after incubation in α-minimum essential medium (α-MEM), was done by X-ray photoelectron spectroscopy (XPS), in order to analyze the insulin behavior at the implant – α-MEM interface. In the absence of serum proteins in cell culture medium, the coated insulin layer remained intact, but experienced a time-dependent structural transformation exposing hydrophobic parts of the protein toward the solution. The presence of fetal bovine serum (FBS) in the medium resulted in partial substitution of insulin by serum proteins. In spite of some insulin release, the remaining coated layer demonstrated a direct surface effect by stabilizing the structure of protein competitors, and by supporting the accumulation of calcium and phosphate ions at the interface. A structurally stable protein layer with incorporated calcium and phosphate ions at the implant–tissue interface could be an important prerequisite for enhanced bone formation.

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