To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Proteomic analyses of lim… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Proteomic analyses of limbic regions in neonatal male, female and androgen receptor knockout mice

Journal article
Authors Anna Zettergren
Sara Karlsson
Erik Studer
Anna Sarvimaki
Petronella Kettunen
Annika Thorsell
Carina Sihlbom
Lars Westberg
Published in Bmc Neuroscience
Volume 18
ISSN 1471-2202
Publication year 2017
Published at Core Facilities, Proteomics
Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
Institute of Neuroscience and Physiology, Department of Pharmacology
Language en
Keywords Proteomics, Sex differences, Androgen receptor, Neonatal, Amygdala, Hypothalamus, sexually dimorphic behaviors, dependent cell-death, sex-differences, gene-expression, mouse-brain, stria terminalis, substrate marcks, preoptic area, mutant mice, bed nucleus, Neurosciences & Neurology
Subject categories Neurology, Neurosciences


Background: It is well-established that organizational effects of sex steroids during early development are fundamental for sex-typical displays of, for example, mating and aggressive behaviors in rodents and other species. Male and female brains are known to differ with respect to neuronal morphology in particular regions of the brain, including the number and size of neurons, and the density and length of dendrites in nuclei of hypothalamus and amygdala. The aim of the present study was to use global proteomics to identify proteins differentially expressed in hypothalamus/amygdala during early development (postnatal day 8) of male, female and conditional androgen receptor knockout (AR(NesDel)) male mice, lacking androgen receptors specifically in the brain. Furthermore, verification of selected sexually dimorphic proteins was performed using targeted proteomics. Results: Our proteomic approach, iTRAQ, allowed us to investigate expression differences in the 2998 most abundantly expressed proteins in our dissected tissues. Approximately 170 proteins differed between the sexes, and 38 proteins between AR(NesDel) and control males (p < 0.05). In line with previous explorative studies of sexually dimorphic gene expression we mainly detected subtle protein expression differences (fold changes < 1.3). The protein MARCKS (myristoylated alanine rich C kinase substrate), having the largest fold change of the proteins selected from the iTRAQ analyses and of known importance for synaptic transmission and dendritic branching, was confirmed by targeted proteomics as differentially expressed between the sexes. Conclusions: Overall, our results provide solid evidence that a large number of proteins show sex differences in their brain expression and could potentially be involved in brain sexual differentiation. Furthermore, our finding of a sexually dimorphic expression of MARCKS in the brain during development warrants further investigation on the involvement in sexual differentiation of this protein.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?