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Measuring ribonucleotide incorporation into DNA in vitro and in vivo.

Chapter in book
Authors Anders R Clausen
Jessica S Williams
Thomas A Kunkel
Published in DNA Replication. Sonya Vengrova, Jacob Dalgaard (red.) (Methods in molecular biology)
Pages 123-39
ISBN 978-1-4939-2595-7
ISSN 1940-6029
Publisher Springer
Publication year 2015
Published at
Pages 123-39
Language en
Links dx.doi.org/10.1007/978-1-4939-2596-...
www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Alkalies, pharmacology, Base Sequence, Biological Assay, methods, DNA, Fungal, metabolism, Electrophoresis, Agar Gel, Genome, Fungal, Molecular Sequence Data, Ribonucleotides, metabolism, Saccharomyces cerevisiae, metabolism, Staining and Labeling
Subject categories Biochemistry and Molecular Biology

Abstract

Ribonucleotides are incorporated into genomes by DNA polymerases, they can be removed, and if not removed, they can have deleterious and beneficial consequences. Here, we describe an assay to quantify stable ribonucleotide incorporation by DNA polymerases in vitro, and an assay to probe for ribonucleotides in each of the two DNA strands of the yeast nuclear genome.

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