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Reaction of complement factors and proteasomes in experimental encephalitis.

Journal article
Authors Stefan Lange
Tomas Bergström
Ewa Johansson
Merna Oshalim
Ivar Lönnroth
Published in Journal of neurovirology
Volume 23
Issue 2
Pages 313–318
ISSN 1538-2443
Publication year 2017
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 313–318
Language en
Keywords Herpes simplex virus type 1, Encephalitis, Complement factor, Proteasome, Compleasome
Subject categories Clinical Laboratory Medicine


Herpes simplex virus type 1 (HSV-1) encephalitis causes a deleterious inflammation and elevated intracranial pressure. As a step towards examining the origin of the inflammation, we here report the response of circulating proteasomes and complement factors in blood and cerebrospinal fluid (CSF) in rats infected with HSV-1. Infection was via the nasal route, with 1.1 × 10(4) plaque-forming units of HSV-1 strain 2762 given in one or both nostrils. A sandwich enzyme-linked immunosorbent assay was used to study the level of 26S proteasomes and their complex formation with complement factors 3 and 4. HSV-1 infection in the rat causes a complex formation between complement factors and proteasomes, which we designate compleasomes. In the first experiment, with HSV-1 given in both nostrils, compleasomes containing complement factors 3 and 4 increased significantly in both blood plasma and CSF. The concentration of proteasomes in plasma was similar in controls and infected rats (320 ± 163 vs. 333 ± 125 ng/ml). In the second experiment, with HSV-1 given in one nostril, CSF levels were 1 ± 1 ng/ml in controls and 56 ± 22 ng/ml in the HSV-1 group, whereas the total protein concentration in CSF remained the same in the two groups. The compleasome response was limited to CSF, with a highly significant difference between infected rats and controls (n = 11, p < 0.001). It was possible to mimic the reaction between proteasomes and complements 3 and 4 in vitro in the presence of ATP.

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