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Isolation and Characterization of Polymorphic Microsatellite DNA Markers from an Amazonian White-sand Vegetation Specialist Bird, Xenopipo atronitens (Aves: Pipridae)

Journal article
Authors C. Gubili
Camila Ritter
J. Motta
I. P. Farias
J. Bates
R. Canton
J. M. G. Capurucho
C. Cornelius
K. A. Feldheim
C. C. Ribas
Published in Wilson Journal of Ornithology
Volume 128
Issue 3
Pages 668-672
ISSN 1559-4491
Publication year 2016
Published at Department of Biological and Environmental Sciences
Pages 668-672
Language en
Links dx.doi.org/10.1676/1559-4491-128.3....
Keywords Amazon, Black Manakin, campina, nuclear markers, Xenopipo atronitens, software, Zoology
Subject categories Biological Sciences

Abstract

Thirteen novel polymorphic microsatellite loci were isolated and characterized from the Amazonian Black Manakin, Xenopipo atronitens. The loci were screened using 30 samples from Brazil. A total of 13 loci were variable with number of alleles per locus ranging from 2 to 17, whilst the observed and expected heterozygosities varied from 0.067-0.900 and 0.310-0.910, respectively. Five loci deviated significantly from the Hardy-Weinberg equilibrium, whereas one pair of loci deviated significantly from linkage disequilibrium. The set of markers will be a useful tool for future population genetic and kinship studies of X. atronitens, an Amazonian white-sand vegetation specialist.

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